Lymph Node Transplantation Results in Spontaneous Lymphatic Reconnection and Restoration of Lymphatic Flow
Identifieur interne : 002919 ( Main/Exploration ); précédent : 002918; suivant : 002920Lymph Node Transplantation Results in Spontaneous Lymphatic Reconnection and Restoration of Lymphatic Flow
Auteurs : Seth Z. Aschen [États-Unis] ; Gina Farias-Eisner [États-Unis] ; Daniel A. Cuzzone [États-Unis] ; Nicholas J. Albano [États-Unis] ; Swapna Ghanta [États-Unis] ; Evan S. Weitman [États-Unis] ; Sagrario Ortega ; Babak J. Mehrara [États-Unis]Source :
- Plastic and reconstructive surgery [ 0032-1052 ] ; 2014.
Descripteurs français
- KwdFr :
- MESH :
English descriptors
- KwdEn :
- MESH :
- physiology : Lymph Nodes, Lymphatic Vessels.
- surgery : Lymphatic Vessels.
- transplantation : Lymph Nodes.
- Animals, Male, Mice, Mice, Inbred C57BL.
Abstract
Although lymph node transplantation has been shown to improve lymphatic function the mechanisms regulating lymphatic vessel reconnection and functional status of lymph nodes remains poorly understood.
We developed and used LacZ lymphatic reporter mice to examine the lineage of lymphatic vessels infiltrating transferred lymph nodes. In addition, we analyzed lymphatic function, expression of vascular endothelial growth factor (VEGF-C), maintenance of T and B cell zone, and anatomic localization of lymphatics and high endothelial venules (HEVs).
Reporter mice were specific and highly sensitive in identifying lymphatic vessels. Lymph node transfer was associated with rapid return of lymphatic function and clearance of Tc99 secondary to a massive infiltration of recipient mouse lymphatics and putative connections to donor lymphatics. T and B cell populations in the lymph node were maintained. These changes correlated with marked increases in the expression of VEGF-C in the perinodal fat and infiltrating lymphatics. Newly formed lymphatic channels in transferred lymph nodes were in close anatomic proximity to HEVs.
Transferred lymph nodes have rapid infiltration of functional host lymphatic vessels and maintain T and B cell populations. This process correlates with increased endogenous expression of VEGF-C in the perinodal fat and infiltrating lymphatics. Anatomic proximity of newly formed lymphatics and HEVs supports the hypothesis that lymph node transfer can improve lymphedema by exchanges with the systemic circulation.
Url:
DOI: 10.1097/01.prs.0000436840.69752.7e
PubMed: 24469165
PubMed Central: 4066306
Affiliations:
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Le document en format XML
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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Lymph Node Transplantation Results in Spontaneous Lymphatic Reconnection and Restoration of Lymphatic Flow</title>
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<author><name sortKey="Farias Eisner, Gina" sort="Farias Eisner, Gina" uniqKey="Farias Eisner G" first="Gina" last="Farias-Eisner">Gina Farias-Eisner</name>
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<country xml:lang="fr">États-Unis</country>
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<wicri:noCountry code="subfield">Spain 28029</wicri:noCountry>
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<author><name sortKey="Mehrara, Babak J" sort="Mehrara, Babak J" uniqKey="Mehrara B" first="Babak J." last="Mehrara">Babak J. Mehrara</name>
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<country xml:lang="fr">États-Unis</country>
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<series><title level="j">Plastic and reconstructive surgery</title>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term>
<term>Lymph Nodes (physiology)</term>
<term>Lymph Nodes (transplantation)</term>
<term>Lymphatic Vessels (physiology)</term>
<term>Lymphatic Vessels (surgery)</term>
<term>Male</term>
<term>Mice</term>
<term>Mice, Inbred C57BL</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term>
<term>Mâle</term>
<term>Noeuds lymphatiques (physiologie)</term>
<term>Noeuds lymphatiques (transplantation)</term>
<term>Souris</term>
<term>Souris de lignée C57BL</term>
<term>Vaisseaux lymphatiques ()</term>
<term>Vaisseaux lymphatiques (physiologie)</term>
</keywords>
<keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Noeuds lymphatiques</term>
<term>Vaisseaux lymphatiques</term>
</keywords>
<keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Lymph Nodes</term>
<term>Lymphatic Vessels</term>
</keywords>
<keywords scheme="MESH" qualifier="surgery" xml:lang="en"><term>Lymphatic Vessels</term>
</keywords>
<keywords scheme="MESH" qualifier="transplantation" xml:lang="en"><term>Lymph Nodes</term>
</keywords>
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<term>Male</term>
<term>Mice</term>
<term>Mice, Inbred C57BL</term>
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<keywords scheme="MESH" xml:lang="fr"><term>Animaux</term>
<term>Mâle</term>
<term>Noeuds lymphatiques</term>
<term>Souris</term>
<term>Souris de lignée C57BL</term>
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<front><div type="abstract" xml:lang="en"><sec id="S1"><title>Background</title>
<p id="P1">Although lymph node transplantation has been shown to improve lymphatic function the mechanisms regulating lymphatic vessel reconnection and functional status of lymph nodes remains poorly understood.</p>
</sec>
<sec id="S2"><title>Methods</title>
<p id="P2">We developed and used LacZ lymphatic reporter mice to examine the lineage of lymphatic vessels infiltrating transferred lymph nodes. In addition, we analyzed lymphatic function, expression of vascular endothelial growth factor (VEGF-C), maintenance of T and B cell zone, and anatomic localization of lymphatics and high endothelial venules (HEVs).</p>
</sec>
<sec id="S3"><title>Results</title>
<p id="P3">Reporter mice were specific and highly sensitive in identifying lymphatic vessels. Lymph node transfer was associated with rapid return of lymphatic function and clearance of Tc<sup>99</sup>
secondary to a massive infiltration of recipient mouse lymphatics and putative connections to donor lymphatics. T and B cell populations in the lymph node were maintained. These changes correlated with marked increases in the expression of VEGF-C in the perinodal fat and infiltrating lymphatics. Newly formed lymphatic channels in transferred lymph nodes were in close anatomic proximity to HEVs.</p>
</sec>
<sec id="S4"><title>Conclusions</title>
<p id="P4">Transferred lymph nodes have rapid infiltration of functional host lymphatic vessels and maintain T and B cell populations. This process correlates with increased endogenous expression of VEGF-C in the perinodal fat and infiltrating lymphatics. Anatomic proximity of newly formed lymphatics and HEVs supports the hypothesis that lymph node transfer can improve lymphedema by exchanges with the systemic circulation.</p>
</sec>
</div>
</front>
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<region><li>État de New York</li>
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</noCountry>
<country name="États-Unis"><region name="État de New York"><name sortKey="Aschen, Seth Z" sort="Aschen, Seth Z" uniqKey="Aschen S" first="Seth Z." last="Aschen">Seth Z. Aschen</name>
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<name sortKey="Albano, Nicholas J" sort="Albano, Nicholas J" uniqKey="Albano N" first="Nicholas J." last="Albano">Nicholas J. Albano</name>
<name sortKey="Cuzzone, Daniel A" sort="Cuzzone, Daniel A" uniqKey="Cuzzone D" first="Daniel A." last="Cuzzone">Daniel A. Cuzzone</name>
<name sortKey="Farias Eisner, Gina" sort="Farias Eisner, Gina" uniqKey="Farias Eisner G" first="Gina" last="Farias-Eisner">Gina Farias-Eisner</name>
<name sortKey="Ghanta, Swapna" sort="Ghanta, Swapna" uniqKey="Ghanta S" first="Swapna" last="Ghanta">Swapna Ghanta</name>
<name sortKey="Mehrara, Babak J" sort="Mehrara, Babak J" uniqKey="Mehrara B" first="Babak J." last="Mehrara">Babak J. Mehrara</name>
<name sortKey="Weitman, Evan S" sort="Weitman, Evan S" uniqKey="Weitman E" first="Evan S." last="Weitman">Evan S. Weitman</name>
</country>
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